Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.740812
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dc.titleUptake of photosensitizers by bacteria is influenced by the presence of cations
dc.contributor.authorKishen, A.
dc.contributor.authorGeorge, S.
dc.date.accessioned2014-09-18T09:23:13Z
dc.date.available2014-09-18T09:23:13Z
dc.date.issued2007
dc.identifier.citationKishen, A., George, S. (2007). Uptake of photosensitizers by bacteria is influenced by the presence of cations. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 6535 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.740812
dc.identifier.isbn0819466573
dc.identifier.issn16057422
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79943
dc.description.abstractThis investigation studies the influence of cations on photosensitizer uptake by Enterococcus faecalis (gram positive) and Actinobacillus actinomycetemcomitans (gram negative). Methods- The uptake of Methylene blue (MB) and Indocyanine Green (ICG), by bacteria were studied under the influence of divalent cations (CaCl2 & MgCl2) and EDTA. Further, E. faecalis cells subjected to trypsinisation and calcium channel blocker (verapamil) were also analysed for MB and ICG uptake inorder to understand the mechanism of photosensitizer uptake. Results- Uptake of ICG was enhanced in the presence of divalent cations in E. faecalis and A. actinomycetemcomitans. Treating cells with EDTA had no significant effect on the photosensitizer uptake, although the highest concentration tested showed an enhancement of uptake. In contrast to ICG, MB showed a decreased uptake by bacterial cells on subjecting them to divalent cations and EDTA. Calcium channel blocker had no significant inhibitory effect on photosensitizers uptake. However, trypsin treatment resulted in significant reduction of ICG uptake. The result suggested that ICG uptake by bacteria is mediated through specific transporter protein while MB is associated with the outer surface structures of bacterial cells.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1117/12.740812
dc.sourceScopus
dc.subjectA. Actinomycetemcomitans
dc.subjectDivalent cations
dc.subjectE. Faecalis
dc.subjectEDTA
dc.subjectIndocyanine green
dc.subjectMethylene blue
dc.typeConference Paper
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.doi10.1117/12.740812
dc.description.sourcetitleProgress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.description.volume6535
dc.description.page-
dc.identifier.isiut000250170600022
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