Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.tiv.2004.08.006
DC FieldValue
dc.titleTooth slice organ culture and established cell line culture models for cytotoxicity assessment of dental materials
dc.contributor.authorSaw, T.Y.
dc.contributor.authorCao, T.
dc.contributor.authorYap, A.U.J.
dc.contributor.authorLee Ng, M.M.
dc.date.accessioned2014-09-18T09:23:02Z
dc.date.available2014-09-18T09:23:02Z
dc.date.issued2005-02
dc.identifier.citationSaw, T.Y., Cao, T., Yap, A.U.J., Lee Ng, M.M. (2005-02). Tooth slice organ culture and established cell line culture models for cytotoxicity assessment of dental materials. Toxicology in Vitro 19 (1) : 145-154. ScholarBank@NUS Repository. https://doi.org/10.1016/j.tiv.2004.08.006
dc.identifier.issn08872333
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79935
dc.description.abstractThe aim was to compare the use of different cell-material contact test methods with two different biological systems (cell line and tooth slice cultures) for cytotoxicity assessment of dental materials. Cytotoxicity of composites polymerized with two halogen-based and two light-emitting diode (LED) light-curing units (LCUs) served as the basis for comparison. Disk shaped specimens (7 x 2 mm) were fabricated using the four light sources. Composites were tested using L-929 cell line using direct/indirect/extract tests in accordance to standard protocols. Cytotoxicity was assessed using neutral red uptake. Tooth slice organ cultures were also employed to test the dental materials using direct/indirect test methods. Histomorphometric cell counting of intact odontoblasts and pulp fibroblasts and the use of tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were applied for cytotoxicity evaluation. Discrepancy in result presentation was observed in the different tests used with L-929. Sensitivity levels of the L-929 tests ranked as follows: extract test < direct contact test < indirect contact test. Tooth slice tests confirmed that L-929 direct contact test proved to be the most reliable test among the three. In conclusion, this study highlights the risk involved when relying on a single test method for cytotoxicity assessment. It would be advisable to test different culture models and then proceed using more clinically relevant biological system that stimulate the in vivo situation for confirmation. © 2004 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.tiv.2004.08.006
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDENTISTRY
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.doi10.1016/j.tiv.2004.08.006
dc.description.sourcetitleToxicology in Vitro
dc.description.volume19
dc.description.issue1
dc.description.page145-154
dc.description.codenTIVIE
dc.identifier.isiut000226888400016
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

36
checked on Sep 17, 2020

WEB OF SCIENCETM
Citations

29
checked on Sep 10, 2020

Page view(s)

79
checked on Sep 13, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.