Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1751-1097.2007.00244.x
DC FieldValue
dc.titleInfluence of photosensitizer solvent on the mechanisms of photoactivated killing of Enterococcus faecalis
dc.contributor.authorGeorge, S.
dc.contributor.authorKishen, A.
dc.date.accessioned2014-09-18T09:22:38Z
dc.date.available2014-09-18T09:22:38Z
dc.date.issued2008-05
dc.identifier.citationGeorge, S., Kishen, A. (2008-05). Influence of photosensitizer solvent on the mechanisms of photoactivated killing of Enterococcus faecalis. Photochemistry and Photobiology 84 (3) : 734-740. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1751-1097.2007.00244.x
dc.identifier.issn00318655
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79909
dc.description.abstractThis study evaluated the mechanisms involved and the influence of photosensitizer solvent in the killing of Enterococcus faecalis using photodynamic therapy (PDT). Enterococcus faecalis cells incubated with 100 μm methylene blue dissolved in water and in MIX (a mixture of glycerol:ethanol: water) were irradiated with 664 nm diode laser (63.69 J cm-2). The effect of PDT on the viability of bacteria, and the functional integrity of cell wall, chromosomal DNA and membrane proteins were analyzed. The bactericidal action of PDT was significantly higher when a MIX-based photosensitizer solvent was used (P < 0.001). Fluorimetric and fluorescence microscopy-based analysis showed the functional impairment of E. faecalis cell wall which was significantly higher when a MIX-based photosensitizer solvent was used (P < 0.001). PDT with MIX-based photosensitizer solvent showed extensive damage to chromosomal DNA. However, both PDT conditions showed similar trend in the degradation of membrane proteins, although cross-linked proteins were evident only in PDT conducted with MIX-based photosensitizer solvent. The findings from our study showed that PDT destroyed the functional integrity of cell wall, DNA and membrane proteins of E. faecalis. The degrees of damage on these targets were influenced by the photosensitizer solvent used during PDT. © 2008 The Authors.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1751-1097.2007.00244.x
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.doi10.1111/j.1751-1097.2007.00244.x
dc.description.sourcetitlePhotochemistry and Photobiology
dc.description.volume84
dc.description.issue3
dc.description.page734-740
dc.description.codenPHCBA
dc.identifier.isiut000255518400028
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

48
checked on May 20, 2022

WEB OF SCIENCETM
Citations

46
checked on May 20, 2022

Page view(s)

136
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.