Please use this identifier to cite or link to this item:
|Title:||Human embryonic stem cell differentiation into odontoblastic lineage: An in vitro study||Authors:||Kidwai, F.K.
Human embryonic stem cells
Mesenchymal stem cells
|Issue Date:||Apr-2014||Citation:||Kidwai, F.K., Movahednia, M.M., Iqbal, K., Jokhun, D.S., Cao, T., Fawzy, A.S. (2014-04). Human embryonic stem cell differentiation into odontoblastic lineage: An in vitro study. International Endodontic Journal 47 (4) : 346-355. ScholarBank@NUS Repository. https://doi.org/10.1111/iej.12150||Abstract:||Aim: The aim of this study was to differentiate human embryonic stem cells (hESCs) into odontoblastic lineage in an optimized culture milieu. Methodology: In Phase 1, hESCs were differentiated into mesenchymal stem cells (H9-MSCs). In Phase 2, H9-MSCs were then differentiated into odontoblast-like cells (H9-Odont) under the stimulation of FGF-8 and BMP-4. Alternatively, H9-MSCs were differentiated into osteogenic lineage (H9-Osteo). In Phase 3, H9-Odont were seeded on 17% EDTA-treated dentine substrates in the presence of FGF-8 and BMP-4 for further differentiation. All experiments were performed in triplicate (n = 3). One-way anova was used to test hESC differentiation into different cell types. Post hoc Tukey's test was used to compare between groups. P||Source Title:||International Endodontic Journal||URI:||http://scholarbank.nus.edu.sg/handle/10635/79908||ISSN:||01432885||DOI:||10.1111/iej.12150|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 25, 2021
WEB OF SCIENCETM
checked on Oct 18, 2021
checked on Oct 14, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.