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Title: Human embryonic stem cell differentiation into odontoblastic lineage: An in vitro study
Authors: Kidwai, F.K.
Movahednia, M.M.
Iqbal, K.
Jokhun, D.S.
Cao, T.
Fawzy, A.S. 
Keywords: Dentine
Growth factors
Human embryonic stem cells
Mesenchymal stem cells
Odontoblastic differentiation
Issue Date: Apr-2014
Citation: Kidwai, F.K., Movahednia, M.M., Iqbal, K., Jokhun, D.S., Cao, T., Fawzy, A.S. (2014-04). Human embryonic stem cell differentiation into odontoblastic lineage: An in vitro study. International Endodontic Journal 47 (4) : 346-355. ScholarBank@NUS Repository.
Abstract: Aim: The aim of this study was to differentiate human embryonic stem cells (hESCs) into odontoblastic lineage in an optimized culture milieu. Methodology: In Phase 1, hESCs were differentiated into mesenchymal stem cells (H9-MSCs). In Phase 2, H9-MSCs were then differentiated into odontoblast-like cells (H9-Odont) under the stimulation of FGF-8 and BMP-4. Alternatively, H9-MSCs were differentiated into osteogenic lineage (H9-Osteo). In Phase 3, H9-Odont were seeded on 17% EDTA-treated dentine substrates in the presence of FGF-8 and BMP-4 for further differentiation. All experiments were performed in triplicate (n = 3). One-way anova was used to test hESC differentiation into different cell types. Post hoc Tukey's test was used to compare between groups. P 
Source Title: International Endodontic Journal
ISSN: 01432885
DOI: 10.1111/iej.12150
Appears in Collections:Staff Publications

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