Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/79589
DC FieldValue
dc.titleCONFORMATIONAL CHANGES OF SINGLE DNA MOLECULES INDUCED BY H-NS AND HU PROTEINS IN A NANOSPACE
dc.contributor.authorDURGARAO GUTTULA
dc.date.accessioned2014-09-04T18:00:11Z
dc.date.available2014-09-04T18:00:11Z
dc.date.issued2014-01-21
dc.identifier.citationDURGARAO GUTTULA (2014-01-21). CONFORMATIONAL CHANGES OF SINGLE DNA MOLECULES INDUCED BY H-NS AND HU PROTEINS IN A NANOSPACE. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79589
dc.description.abstractTHE EFFECTS OF NUCLEOID-ASSOCIATED PROTEINS H-NS AND HU ON THE CONFORMATION OF SINGLE DNA MOLECULES IN A NANOSPACE WERE INVESTIGATED WITH FLUORESCENCE MICROSCOPY. FOR H-NS PROJECT, TEMPORAL CONFORMATION CHANGES WERE ALSO INVESTIGATED USING CROSS CHANNELS. THE CONCENTRATION OF PROTEIN INCREASES, THE DNA-HNS COMPLEX SHOWS AN INCREASE IN LENGTH AT LOW IONIC STRENGTH, AND A DECREASE IN LENGTH AT HIGH IONIC STRENGTH. ON THE OTHER HAND, DNA-HU COMPLEX SHOWS CONTRACTION BY BRIDGING IN HIGH AND LOW IONIC STRENGTH. CONFINEMENT IN A NANOCHANNEL ALSO FACILITATES THE COMPACTION OF DNA INTO A CONDENSED FORM FOR OVER-THRESHOLD CONCENTRATIONS OF PROTEINS. MAGNESIUM FACILITATES BUT ARE NOT REQUIRED FOR BRIDGING NOR CONDENSATION. OUR RESULTS SHOW THAT THE EFFECTS OF NAPS ON THE CONFORMATION AND FOLDING OF DNA DO NOT DEPEND ON DNA-PROTEIN INTERACTION AND CROWDING PER SE, BUT THE INTERPLAY WITH THE CONFINEMENT IN A NANOSPACE SUCH AS WITHIN A BACTERIAL CELL IS OF PARAMOUNT IMPORTANCE.
dc.language.isoen
dc.subjectNanochannels, DNA, H-NS, HU, filamentation, bridging
dc.typeThesis
dc.contributor.departmentPHYSICS
dc.contributor.supervisorJOHAN R C VAN DER MAAREL
dc.contributor.supervisorVAN KAN, JEROEN ANTON
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
GuttulaDurgaraoDG.PDF12.91 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

110
checked on May 21, 2019

Download(s)

29
checked on May 21, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.