Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0039-9140(97)00288-9
Title: The degree of deacetylation of chitosan: Advocating the first derivative UV-spectrophotometry method of determination
Authors: Tan, S.C.
Khor, E. 
Tan, T.K. 
Wong, S.M. 
Keywords: Chitosan
Degree of deacetylation
First derivative UV-spectrophotometry
Issue Date: Feb-1998
Citation: Tan, S.C., Khor, E., Tan, T.K., Wong, S.M. (1998-02). The degree of deacetylation of chitosan: Advocating the first derivative UV-spectrophotometry method of determination. Talanta 45 (4) : 713-719. ScholarBank@NUS Repository. https://doi.org/10.1016/S0039-9140(97)00288-9
Abstract: The degree of deacetylation (DD) is increasingly becoming an important property for chitosan, as it determines how the biopolymer can be applied. Therefore, a simple, rapid and reliable method of determining the DD for chitosan is essential. In this report, the DD of chitosan was determined by nuclear magnetic resonance (NMR), linear potentiometric titration (LPT), ninhydrin test and first derivative UV-spectrophotometry (1DUVS). The DD was calculated on a per mol basis instead of on a per mass basis. This is important as the molecular weights of N-acetyl-D-glucosamine and D-glucosamine are different. By converting the mass of N-acetyl-D-glucosamine and D-glucosamine into mols and calculating for the percentage of D-glucosamine present in the chitosan sample, a more accurate estimation of the DD can be obtained. Of the four methods, there is good correlation between 1DUVS and NMR. The concentration of chitosan solution for 1DUVS analysis was standardised as 0.1000 mg chitosan per ml of 0.0100 M acetic acid solution. The presence of D-glucosamine was corrected for by a reference curve for N-acetyl-D-glucosamine. 1DUVS is easy to perform, sensitive and the interference of other contaminants to the results is minimal compared with the other three methods. Therefore, we advocate 1DUVS to be used as the standard methods for routine determination of DD of chitosan.
Source Title: Talanta
URI: http://scholarbank.nus.edu.sg/handle/10635/77496
ISSN: 00399140
DOI: 10.1016/S0039-9140(97)00288-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

167
checked on Dec 2, 2019

WEB OF SCIENCETM
Citations

160
checked on Nov 25, 2019

Page view(s)

50
checked on Dec 1, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.