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Title: Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells
Authors: Gomathy, S.S.
Stylianou, C.
Phang, I.Y.
Cool, S.
Nurcombe, V.
Ample, F.
Lear, M.J. 
Gorelik, S.
Hobley, J.
Issue Date: 2010
Citation: Gomathy, S.S.,Stylianou, C.,Phang, I.Y.,Cool, S.,Nurcombe, V.,Ample, F.,Lear, M.J.,Gorelik, S.,Hobley, J. (2010). Raman mapping glucose metabolism during adipogenesis from human mesenchymal stem cells. 2010 Photonics Global Conference, PGC 2010 : -. ScholarBank@NUS Repository.
Abstract: Raman mapping was used to determine the distribution of fats inside of adipocytes and human mesenchymal stem cells during adipogenesis. This proved to be a successful way of mapping the lipid distribution within the cells because of the strong C-H stretching bands of the fats inside the lipid droplets. Furthermore using deuterated glucose in the cell culture medium it was possible to distinguish any fats in droplets or in the cytoplasm that were formed before and after adipogenesis was initiated, because those formed after displayed both C-H and C-D stretching bands. The distributions of the initial metabolites of glucose and the resulting fats in the lipid drops could also be mapped in this way.
Source Title: 2010 Photonics Global Conference, PGC 2010
ISBN: 9781424498826
DOI: 10.1109/PGC.2010.5705978
Appears in Collections:Staff Publications

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