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Title: Optical detection of photo-activated protein using Brewster angle microscopy
Authors: Li, Y.
Gorelik, S.
Tomczak, N.
Song, H.Y. 
Lear, M.J. 
Hobley, J.
Issue Date: 2008
Citation: Li, Y.,Gorelik, S.,Tomczak, N.,Song, H.Y.,Lear, M.J.,Hobley, J. (2008). Optical detection of photo-activated protein using Brewster angle microscopy. 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008 : -. ScholarBank@NUS Repository.
Abstract: Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).
Source Title: 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
ISBN: 9781424429059
DOI: 10.1109/IPGC.2008.4781381
Appears in Collections:Staff Publications

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