Please use this identifier to cite or link to this item:
|Title:||Analysis of Chinese medicine preparations by capillary electrophoresis- mass spectrometry||Authors:||Feng, H.-T.
|Issue Date:||3-Oct-2003||Citation:||Feng, H.-T., Yuan, L.-L., Li, S.F.Y. (2003-10-03). Analysis of Chinese medicine preparations by capillary electrophoresis- mass spectrometry. Journal of Chromatography A 1014 (1-2) : 83-91. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(03)00942-7||Abstract:||In Chinese medicines, herbs are usually prepared before use by patients. Since the preparation procedures convert the original component into one or more products, study of the procedures is usually complex and involves several compounds. On-line coupling of capillary electrophoresis (CE) to mass spectrometry (MS) allows both the efficient separation of CE and the specific and sensitive detection of MS to be achieved. In this study, CE-MS was applied to the determination of alkaloids in Maqianzi (the seed of Strychnos pierrian) and Wutou (aconite root, Radix aconiti praeparata) during the preparation procedure. With optimal CE-MS conditions, alkaloids in both prepared and unprepared Maqianzi were determined successfully in the total ion current (TIC) mode. However, single ion monitoring (SIM) had to be applied for the separation of aconitum alkaloids and their hydrolysis products. Quantification data indicated that MS detection under SIM mode is more sensitive than UV detection. Based on the CE-MS method developed, the hydrolysis of aconitum alkaloids in water and methanol was also studied. © 2003 Published by Elsevier B.V.||Source Title:||Journal of Chromatography A||URI:||http://scholarbank.nus.edu.sg/handle/10635/77397||ISSN:||00219673||DOI:||10.1016/S0021-9673(03)00942-7|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 16, 2019
WEB OF SCIENCETM
checked on Oct 8, 2019
checked on Oct 12, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.