Please use this identifier to cite or link to this item:
Title: Visual detection of DNA from salmonella and mycobacterium using split DNAzymes
Authors: Darius, A.K.L.
Ling, N.J.
Mahesh, U. 
Issue Date: 2010
Citation: Darius, A.K.L., Ling, N.J., Mahesh, U. (2010). Visual detection of DNA from salmonella and mycobacterium using split DNAzymes. Molecular BioSystems 6 (5) : 792-794. ScholarBank@NUS Repository.
Abstract: The split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Most reports on its use have however been constrained to proof-of-concept demonstrations using synthetic oligonucleotides. This is due to the inherent complexities of the assay, and the multiple components involved. Herein, we have overcome several of these challenges and have successfully integrated asymmetric PCR with the visual detection step, allowing enriched targets from complex samples to be conveniently detected. This workflow would enable the DNAzyme system to be applied for point-of-care DNA detection applications. We have established the platform herein as a simple and robust method to determine the presence of target DNA sequences post-PCR (as required for pathogen detection), without the need for gels or other apparatus. Nanomolar concentrations of amplified targets were detected using the workflow established, enabling the detection of salmonella and mycobacterium targets through a color change reaction, observable just by eye. © 2010 The Royal Society of Chemistry.
Source Title: Molecular BioSystems
ISSN: 1742206X
DOI: 10.1039/c001923b
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 30, 2020


checked on Oct 22, 2020

Page view(s)

checked on Oct 23, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.