The efficacy of a dual anticalcification agent treatment in the prevention of calcification of glutaraldehyde-fixed porcine pericardium

Abstract

The efficacy of a series of combined anticalcification treatments utilizing a chloroform-methanol mixture or a surfactant, sodium dodecyl sulfate, followed by trivalent metal ion Fe(III) were evaluated with glutaraldehyde-treated porcine pericardium. The results indicate that the combined treatments are comparable in the reduction of calcification levels to that of the metal ion alone. Furthermore, pretreatment with the organic reagents improves the residence time of Fe(III) in the tissue. The results suggest that the offers a basis for improved mitigation of calcification in glutaraldehyde-treated tissue over that so far found in single systems.