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Title: Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing
Authors: Girish, A.
Sun, H.
Yeo, D.S.Y.
Chen, G.Y.J. 
Chua, T.-K. 
Yao, S.Q. 
Keywords: Intein
N-terminal cysteine-containing protein
Protein array
Issue Date: 16-May-2005
Citation: Girish, A., Sun, H., Yeo, D.S.Y., Chen, G.Y.J., Chua, T.-K., Yao, S.Q. (2005-05-16). Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing. Bioorganic and Medicinal Chemistry Letters 15 (10) : 2447-2451. ScholarBank@NUS Repository.
Abstract: One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. © 2005 Elsevier Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
ISSN: 0960894X
DOI: 10.1016/j.bmcl.2005.03.079
Appears in Collections:Staff Publications

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