Please use this identifier to cite or link to this item: https://doi.org/10.1039/b904060a
Title: Practical synthesis of maleimides and coumarin-linked probes for protein and antibody labelling via reduction of native disulfides
Authors: Song, H.Y. 
Ngai, M.H. 
Song, Z.Y.
MacAry, P.A.
Hobley, J.
Lear, M.J. 
Issue Date: 2009
Citation: Song, H.Y., Ngai, M.H., Song, Z.Y., MacAry, P.A., Hobley, J., Lear, M.J. (2009). Practical synthesis of maleimides and coumarin-linked probes for protein and antibody labelling via reduction of native disulfides. Organic and Biomolecular Chemistry 7 (17) : 3400-3406. ScholarBank@NUS Repository. https://doi.org/10.1039/b904060a
Abstract: The cellular tracking, detection and sensing of protein or antibody movement are important aspects to advance our understanding of biomolecular interactions and activity. Antibodies modified with fluorescent dyes are also valuable tools, especially in immunology research. We describe here a proof-of-principle study of a new water-soluble coumarin probe with a maleimide thiol-reacting unit to fluorescently tag biomolecules. Highlights include: (1) a convenient water-based preparation of N-substituted maleimides, (2) a one-pot preparation of activated maleimido-esters, and (3) a bio-conjugation protocol for the selenol-promoted reduction of native disulfide bonds and the 'site-specific' labelling of antibodies with no significant loss of activity. © 2009 The Royal Society of Chemistry.
Source Title: Organic and Biomolecular Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/76794
ISSN: 14770520
DOI: 10.1039/b904060a
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