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|Title:||Optimization of separation of porphyrins by micellar electrokinetic chromatography using the overlapping resolution mapping scheme||Authors:||Yao, Y.J.
|Issue Date:||14-May-1993||Citation:||Yao, Y.J.,Lee, H.K.,Li, S.F.Y. (1993-05-14). Optimization of separation of porphyrins by micellar electrokinetic chromatography using the overlapping resolution mapping scheme. Journal of Chromatography A 637 (2) : 195-200. ScholarBank@NUS Repository.||Abstract:||The capillary electrophoretic separation of nine porphyrins having two to eight carboxylic acid side-chains was optimized by using a systematic optimization scheme based on the overlapping resolution mapping method. Three parameters were considered for the optimization, viz., surfactant (sodium dodecyl sulphate) concentration, amount of organic modifier (N,N-dimethyl-formamide) and the ionic strength of the buffer. To utilize the scheme, a set of seven preplanned experiments were performed. Optimum separation conditions were determined which provided satisfactory separation of the porphyrins within an analysis time of ca. 30 min. © 1993.||Source Title:||Journal of Chromatography A||URI:||http://scholarbank.nus.edu.sg/handle/10635/76691||ISSN:||00219673|
|Appears in Collections:||Staff Publications|
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