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Title: NIR light controlled photorelease of siRNA and its targeted intracellular delivery based on upconversion nanoparticles
Authors: Yang, Y.
Liu, F.
Liu, X. 
Xing, B.
Issue Date: 7-Jan-2013
Citation: Yang, Y., Liu, F., Liu, X., Xing, B. (2013-01-07). NIR light controlled photorelease of siRNA and its targeted intracellular delivery based on upconversion nanoparticles. Nanoscale 5 (1) : 231-238. ScholarBank@NUS Repository.
Abstract: The most notable role of small interfering RNA (siRNA) is in RNA interference (RNAi) and post-transcriptional gene silencing, which leads to a surge of interest in RNAi for both biomedical research and therapeutic applications. However, "naked" siRNA cannot cross cellular membranes freely because of highly negative charges which limits its utility for gene therapy. In this work, a system of near-infrared (NIR) light-induced siRNA release from silica coated upconversion nanoparticles (Si-UCNPs) is presented. These Si-UCNPs were functionalized with cationic photocaged linkers through covalent bonding, which could effectively adsorb anionic siRNA through electrostatic attractions and were easily internalized by living cells. Upon NIR light irradiation, the photocaged linker on the Si-UCNPs surface could be cleaved by the upconverted UV light and thus initiated the intracellular release of the siRNA. The in vitro agarose gel electrophoresis and intracellular imaging results indicated that the Si-UCNPs-based gene carrier system allowed effective siRNA delivery and the applications of NIR light instead of direct high energy UV irradiation may greatly guarantee less cell damage. © 2013 The Royal Society of Chemistry.
Source Title: Nanoscale
ISSN: 20403364
DOI: 10.1039/c2nr32835f
Appears in Collections:Staff Publications

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