Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2005.02.019
DC FieldValue
dc.titleNanodroplet profiling of enzymatic activities in a microarray
dc.contributor.authorUttamchandani, M.
dc.contributor.authorHuang, X.
dc.contributor.authorChen, G.Y.J.
dc.contributor.authorYao, S.Q.
dc.date.accessioned2014-06-23T05:44:55Z
dc.date.available2014-06-23T05:44:55Z
dc.date.issued2005-04-15
dc.identifier.citationUttamchandani, M., Huang, X., Chen, G.Y.J., Yao, S.Q. (2005-04-15). Nanodroplet profiling of enzymatic activities in a microarray. Bioorganic and Medicinal Chemistry Letters 15 (8) : 2135-2139. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2005.02.019
dc.identifier.issn0960894X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/76614
dc.description.abstractWe describe a generic method for the large-scale functional characterization of enzymes in a microarray. Poly-l-lysine and amine reactive slides were coated with fluorogenic substrates sensitive to proteases and phosphatases. Patterning enzymes on the slides by robotic printing produced spatially addressable, segregated droplets that were simultaneously exposed to the on-chip sensors. Multiple enzymes were profiled using this system that provided fluorescence readouts across temporal and stoichiometric dimensions concurrently on a single microarray substrate. This integrated microarray platform is applicable not only for the functional annotation of proteins, but also for the rapid agonist and antagonist discovery and in performing on-chip kinetics. © 2005 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bmcl.2005.02.019
dc.sourceScopus
dc.subjectCoumarin
dc.subjectEnzyme
dc.subjectFluorescence
dc.subjectMicroarray
dc.subjectProtease
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/j.bmcl.2005.02.019
dc.description.sourcetitleBioorganic and Medicinal Chemistry Letters
dc.description.volume15
dc.description.issue8
dc.description.page2135-2139
dc.description.codenBMCLE
dc.identifier.isiut000228432900036
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