Please use this identifier to cite or link to this item:
|Title:||Minimization of Sample Discrimination Introduced by On-Column Fracture/Electrokinetic Injection in Capillary Electrophoresis||Authors:||Wei, H.
|Issue Date:||1-Jun-1998||Citation:||Wei, H.,Ang, K.C.,Li, S.F.Y. (1998-06-01). Minimization of Sample Discrimination Introduced by On-Column Fracture/Electrokinetic Injection in Capillary Electrophoresis. Analytical Chemistry 70 (11) : 2248-2253. ScholarBank@NUS Repository.||Abstract:||Using on-column fracture/electrokinetic injection for sample introduction in capillary electrophoresis (CE) is thought to be a method of no-discrimination electrokinetic injection. However, in this study we found that significant discrimination was observed when injecting samples dissolved in deionized water with the above method. In addition, the discrimination is reverse to that in conventional electrokinetic injection, that is, the less mobile species are injected in larger quantities than the more mobile components. The reason for these phenomena and approaches to reduce the discrimination were studied. Equivalent circuits were established and used to analyze the discrimination under different conditions through computer simulation. The experimental results showed good agreement with the results of the computer simulations. Finally, an on-column fracture/electrokinetic injection method using ramped injection voltage was proposed and shown to be suitable for sample solutions with different conductivity. This method does not cause significant error for practical quantification, even without correcting for the discrimination, because its discrimination is very small.||Source Title:||Analytical Chemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/76511||ISSN:||00032700|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 30, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.