Please use this identifier to cite or link to this item:
Title: Fluorescent approach to quantitation of reactive oxygen species in mainstream cigarette smoke
Authors: Ou, B.
Huang, D. 
Issue Date: 1-May-2006
Citation: Ou, B., Huang, D. (2006-05-01). Fluorescent approach to quantitation of reactive oxygen species in mainstream cigarette smoke. Analytical Chemistry 78 (9) : 3097-3103. ScholarBank@NUS Repository.
Abstract: A novel approach to monitoring of mainstream smoke reactive oxygen species (ROS) has been developed and applied to the quantitation of smoke oxidants. Redoxactive fluorescent probe dihydrorhodamine 6G (DHR-6G) was selected as the molecular probe because it is sensitive to typical smoke ROS. The experimental system couples an automatic cigarette smoke machine fiber-optic fluorometer for real-time monitoring of the reaction progress between cigarette smoke and DHR-6G. Quantitation was achieved based on the amount of rhodamine 6G, which is the sole product from DHR-6G oxidation. With the optimization of the trapping efficiency, we detected 391 nmol of ROS/cigarette in the mainstream CS for a standard cigarette 2R4F under standard Federal Trade Commission smoking protocol. Applying this method, we quantified the ROS of selected cigarettes and found that the cigarettes made of burley tobacco have much (∼10 times) higher ROS content in the smoke than that in the tobacco made of bright tobacco. The smokeless cigarette, Eclipse, has comparable ROS with cigarettes made of bright tobacco. © 2006 American Chemical Society.
Source Title: Analytical Chemistry
ISSN: 00032700
DOI: 10.1021/ac051993s
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Sep 22, 2020


checked on Sep 22, 2020

Page view(s)

checked on Sep 27, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.