Please use this identifier to cite or link to this item: https://doi.org/10.1038/nprot.2011.350
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dc.titleEmbryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1
dc.contributor.authorKang, N.-Y.
dc.contributor.authorYun, S.-W.
dc.contributor.authorHa, H.-H.
dc.contributor.authorPark, S.-J.
dc.contributor.authorChang, Y.-T.
dc.date.accessioned2014-06-23T05:38:25Z
dc.date.available2014-06-23T05:38:25Z
dc.date.issued2011-06
dc.identifier.citationKang, N.-Y., Yun, S.-W., Ha, H.-H., Park, S.-J., Chang, Y.-T. (2011-06). Embryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1. Nature Protocols 6 (7) : 1044-1052. ScholarBank@NUS Repository. https://doi.org/10.1038/nprot.2011.350
dc.identifier.issn17542189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/76089
dc.description.abstractDetecting and isolating specific types of cells is crucial to understanding a variety of biological processes, including development, aging, regeneration and pathogenesis; this understanding, in turn, allows the use of cells for therapeutic purposes, for which stem cells have emerged recently as invaluable materials. The current methods of isolation and characterization of stem cells depend on cell morphology in culture or on immunostaining of specific markers. These methods are, however, time consuming and involve the use of antibodies that may often make the cells unsuitable for further study. We recently developed a fluorescent small molecule named CDy1 (compound of designation yellow 1) that selectively stains live embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). This protocol describes detailed procedures for staining ESC and iPSC in live conditions and for fluorescence-activated cell sorting (FACS) of ESC using CDy1. Cell staining, image acquisition and FACS can be done within 6 h. © 2011 Nature America, Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/nprot.2011.350
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1038/nprot.2011.350
dc.description.sourcetitleNature Protocols
dc.description.volume6
dc.description.issue7
dc.description.page1044-1052
dc.identifier.isiut000292244800010
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