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Title: cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor
Authors: Ng, J.F.
Jaenicke, S. 
Eisele, K.
Dorn, J.
Weil, T.
Issue Date: Sep-2010
Citation: Ng, J.F., Jaenicke, S., Eisele, K., Dorn, J., Weil, T. (2010-09). cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor. Biointerphases 5 (3) : FA41-FA47. ScholarBank@NUS Repository.
Abstract: Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin (cBSA-147) has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column (inside diameter of 530 μm and L=30 m) and evaluated in the enantioselective reduction of ethyl acetoacetate to R-(-)ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization. © 2010 American Vacuum Society.
Source Title: Biointerphases
ISSN: 19348630
DOI: 10.1116/1.3474475
Appears in Collections:Staff Publications

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