Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200600270
Title: Carbon nanotube-enhanced separation of DNA fragments by a portable capillary electrophoresis system with contactless conductivity detection
Authors: Xu, Y.
Li, S.F.Y. 
Keywords: Capillary electrophoresis
Carbon nanotubes
Contactless conductivity detection
DNA fragment
Issue Date: Oct-2006
Citation: Xu, Y., Li, S.F.Y. (2006-10). Carbon nanotube-enhanced separation of DNA fragments by a portable capillary electrophoresis system with contactless conductivity detection. Electrophoresis 27 (20) : 4025-4028. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200600270
Abstract: It was demonstrated that separation of DNA fragments by a CE-contactless conductivity detection system (CE-CCD) could be enhanced with multiple-wall carbon nanotubes (MWCNs) as buffer additive. For HaeIII digest of ΦX174 DNA, optimized MWCN concentration was obtained when the MWCN was above its threshold concentration, at which MWCN could form a network in the buffer as pseudostationary phase to provide additional interaction sites. In the case of larger DNA, MWCN near or below its threshold concentration was enough to provide great improvement of the resolution, which was shown by the separation of the 2-Log DNA ladder. Furthermore, the buffer containing MWCN could provide a more stable baseline in the CE-CCD system, owing to less fluctuation of its conductivity. Compared with CE-UV, CE-CCD with MWCN could provide lower LODs as well as better resolution. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhelm.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/75693
ISSN: 01730835
DOI: 10.1002/elps.200600270
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