Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200290028
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dc.titleAn ionic liquid coating for determination of sildenafil and UK-103,320 in human serum by capillary zone electrophoresis-ion trap mass spectrometry
dc.contributor.authorQin, W.
dc.contributor.authorLi, S.F.Y.
dc.date.accessioned2014-06-23T05:31:55Z
dc.date.available2014-06-23T05:31:55Z
dc.date.issued2002-12
dc.identifier.citationQin, W., Li, S.F.Y. (2002-12). An ionic liquid coating for determination of sildenafil and UK-103,320 in human serum by capillary zone electrophoresis-ion trap mass spectrometry. Electrophoresis 23 (24) : 4110-4116. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200290028
dc.identifier.issn01730835
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/75567
dc.description.abstractIonic liquid (IL) was covalently bonded onto the silica capillary surface and the electroosmotic flow was reversed over a pH range of 3.5 to 7. Sildenafil (SL) and its metabolite UK-103,320 (UK) in human serum were detected by solid-phase extraction followed by capillary zone electrophoresis-mass spectrometry analysis. The running buffer contained 10 mM acetic acid adjusted to pH 4.5 with 1 M ammonia, and the separating voltage was set to -25 kV. The adsorption of the analytes onto the bare capillary wall was eliminated by the IL coating and the drugs were baseline-separated within 14 min with detection limits (S/N = 3) of 14 and 17 ng/mL for SL and UK, respectively. The method developed showed good intraday precision in terms of relative standard deviation (RSD) with respect to migration time (RSD ≤ 0.76%, n = 5) and peak area (RSD ≤ 3.4%, n = 5); the relative coefficients of linear regression for both targets were better than 0.99 within linear dynamic range (SL: 50-600 ng/mL; UK: 40-400 ng/mL). Satisfactory interday precision was obtained with respect to the slope of the regression (RSD ≤ 4.21%, n = 9) and the migration time (RSD ≤ 1.1 %, n = 9). The IL coating could be used with stable electroosmotic flow (RSD = 2.78%, n = 10) for at least 80 h.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/elps.200290028
dc.sourceScopus
dc.subjectCapillary zone electrophoresis
dc.subjectIonic liquid
dc.subjectMass spectrometry
dc.subjectSildenafil (Viagra)
dc.subjectSildenafil metabolite(UK-103,320)
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1002/elps.200290028
dc.description.sourcetitleElectrophoresis
dc.description.volume23
dc.description.issue24
dc.description.page4110-4116
dc.description.codenELCTD
dc.identifier.isiut000180354200009
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