Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00216-013-7155-z
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dc.titleA two-step stimulus-response cell-SELEX method to generate a DNA aptamer to recognize inflamed human aortic endothelial cells as a potential in vivo molecular probe for atherosclerosis plaque detection
dc.contributor.authorJi, K.
dc.contributor.authorLim, W.S.
dc.contributor.authorLi, S.F.Y.
dc.contributor.authorBhakoo, K.
dc.date.accessioned2014-06-23T05:31:07Z
dc.date.available2014-06-23T05:31:07Z
dc.date.issued2013-08
dc.identifier.citationJi, K., Lim, W.S., Li, S.F.Y., Bhakoo, K. (2013-08). A two-step stimulus-response cell-SELEX method to generate a DNA aptamer to recognize inflamed human aortic endothelial cells as a potential in vivo molecular probe for atherosclerosis plaque detection. Analytical and Bioanalytical Chemistry 405 (21) : 6853-6861. ScholarBank@NUS Repository. https://doi.org/10.1007/s00216-013-7155-z
dc.identifier.issn16182642
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/75500
dc.description.abstractAptamers are single-stranded oligonucleotides that are capable of binding wide classes of targets with high affinity and specificity. Their unique three-dimensional structures present numerous possibilities for recognizing virtually any class of target molecules, making them a promising alternative to antibodies used as molecular probes in biomedical analysis and clinical diagnosis. In recent years, cell-systematic evolution of ligands by exponential enrichment (SELEX) has been used extensively to select aptamers for various cell targets. However, aptamers that have evolved from cell-SELEX to distinguish the "stimulus-response cell" have not previously been reported. Moreover, a number of cumbersome and time-consuming steps involved in conventional cell-SELEX reduce the efficiency and efficacy of the aptamer selection. Here, we report a "two-step" methodology of cell-SELEX that successfully selected DNA aptamers specifically against "inflamed" endothelial cells. This has been termed as stimulus-response cell-SELEX (SRC-SELEX). The SRC-SELEX enables the selection of aptamers to distinguish the cells activated by stimulus of healthy cells or cells isolated from diseased tissue. We report a promising aptamer, N55, selected by SRC-SELEX, which can bind specifically to inflamed endothelial cells both in cell culture and atherosclerotic plaque tissue. This aptamer probe was demonstrated as a potential molecular probe for magnetic resonance imaging to target inflamed endothelial cells and atherosclerotic plaque detection. [Figure not available: see fulltext.] © 2013 Springer-Verlag Berlin Heidelberg.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s00216-013-7155-z
dc.sourceScopus
dc.subjectAptamer
dc.subjectAtherosclerosis
dc.subjectInflammation
dc.subjectMRI
dc.subjectSELEX
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1007/s00216-013-7155-z
dc.description.sourcetitleAnalytical and Bioanalytical Chemistry
dc.description.volume405
dc.description.issue21
dc.description.page6853-6861
dc.description.codenABCNB
dc.identifier.isiut000322705600025
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