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Title: A highly sensitive and selective homogenous assay for profiling microRNA expression
Authors: Deng, H.
Shen, W.
Ren, Y.
Gao, Z. 
Keywords: 3,3',5,5'-Tetramethylbenzidine
Duplex-specific nuclease
Issue Date: 15-Apr-2014
Citation: Deng, H., Shen, W., Ren, Y., Gao, Z. (2014-04-15). A highly sensitive and selective homogenous assay for profiling microRNA expression. Biosensors and Bioelectronics 54 : 650-655. ScholarBank@NUS Repository.
Abstract: A highly sensitive and selective homogeneous microRNA (miRNA) assay is described in this report. It is based on the cleavage of DNAzyme moieties from miRNA-hybridized DNAzyme-capped capture probes (DZ-CPs) from magnetic beads by a duplex-specific nuclease (DSN). After removing the magnetic beads together with the unreacted DZ-CPs by using a permanent magnet, amplified colorimetric detection of the target miRNA is realized through the cleaved DNAzyme moieties-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine. The exceptional amplification power of the DSN and the cumulative nature of the signal generation process permit the colorimetric detection of miRNAs down to subfemtomolar levels. The isothermal amplification scheme together with the simple assay protocol makes direct profiling miRNA in real-world samples possible. © 2013 Elsevier B.V.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/j.bios.2013.11.046
Appears in Collections:Staff Publications

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