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Title: Directed transport in renal proximal tubule cells
Authors: Foo, S.E.
Kundu, A.
Lim, H.Y.
Wong, K.P.
Roy, P. 
Keywords: Directed transport
mathematical model
nitrocellulose membrane
uric acid
Issue Date: 2010
Citation: Foo, S.E.,Kundu, A.,Lim, H.Y.,Wong, K.P.,Roy, P. (2010). Directed transport in renal proximal tubule cells. IFMBE Proceedings 31 IFMBE : 744-747. ScholarBank@NUS Repository.
Abstract: A method is proposed to quantify the directed transport flux resulting from a monolayer of polarized proximal tubule cells seeded onto a microporous membrane. The experimental system is comprised of two well mixed fluidic chambers sandwiching the membrane-cell construct. A mathematical model is developed to describe active and passive transport independently across the cell monolayer. The model is comprised of three coupled ordinary differential equations describing the transported solute concentrations in the two chambers and the cell layer. The main parameters in the model are the membrane permeability, cell layer permeability and transporter constants. An analytical solution is derived for the linear equations arising from the assumption of first order transporter activity. The method is more direct as it does not require application of inhibitors of transporter activity, and thereby reduces the number of experiments and simplifies data interpretation. Some limiting features are also discussed. © 2010 International Federation for Medical and Biological Engineering.
Source Title: IFMBE Proceedings
ISBN: 9783540790389
ISSN: 16800737
DOI: 10.1007/978-3-642-14515-5_190
Appears in Collections:Staff Publications

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