Please use this identifier to cite or link to this item:
https://doi.org/10.1002/btpr.175
DC Field | Value | |
---|---|---|
dc.title | Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase | |
dc.contributor.author | Nian, R. | |
dc.contributor.author | Kim, D.S. | |
dc.contributor.author | Tan, L. | |
dc.contributor.author | Kim, C.-W. | |
dc.contributor.author | Choe, W.-S. | |
dc.date.accessioned | 2014-06-19T06:16:13Z | |
dc.date.available | 2014-06-19T06:16:13Z | |
dc.date.issued | 2009-07 | |
dc.identifier.citation | Nian, R., Kim, D.S., Tan, L., Kim, C.-W., Choe, W.-S. (2009-07). Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase. Biotechnology Progress 25 (4) : 1078-1085. ScholarBank@NUS Repository. https://doi.org/10.1002/btpr.175 | |
dc.identifier.issn | 87567938 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/74771 | |
dc.description.abstract | The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperonemediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes. © 2009 American Institute of Chemical Engineers. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/btpr.175 | |
dc.source | Scopus | |
dc.subject | Malate dehydrogenase (MDH) | |
dc.subject | Molecular bichaperone | |
dc.subject | Polyethylene glycol (PEG) | |
dc.subject | Protein refolding | |
dc.type | Conference Paper | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/btpr.175 | |
dc.description.sourcetitle | Biotechnology Progress | |
dc.description.volume | 25 | |
dc.description.issue | 4 | |
dc.description.page | 1078-1085 | |
dc.description.coden | BIPRE | |
dc.identifier.isiut | 000269403000021 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.