Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-3-540-69367-3-10
DC FieldValue
dc.titleSilk-based scaffold for ligament tissue engineering
dc.contributor.authorLiu, H.
dc.contributor.authorFan, H.
dc.contributor.authorWong, E.J.W.
dc.contributor.authorToh, S.L.
dc.contributor.authorGoh, J.C.H.
dc.date.accessioned2014-06-19T05:40:03Z
dc.date.available2014-06-19T05:40:03Z
dc.date.issued2008
dc.identifier.citationLiu, H.,Fan, H.,Wong, E.J.W.,Toh, S.L.,Goh, J.C.H. (2008). Silk-based scaffold for ligament tissue engineering. IFMBE Proceedings 20 IFMBE : 34-37. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-3-540-69367-3-10" target="_blank">https://doi.org/10.1007/978-3-540-69367-3-10</a>
dc.identifier.isbn9783540693666
dc.identifier.issn16800737
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/73844
dc.description.abstractIn recent years, silk has been increasingly studied as the scaffold for ligament tissue engineering due to the biocompatibility, slow degradability, and remarkable mechanical properties. Braided silk scaffold modified with short polypeptide also significantly increases collagen synthesis on it. To increase cell attachment and tissue infiltration, the braided scaffold can be incorporated with silk-gelatin microsponges. A novel silk cable-reinforced gelatin/silk fibroin hybrid scaffold was fabricated, which, apart from providing proper mechanical strength and enlarged surface area, also supported the proliferation and differentiation of MSCs on it. The knitted silk mesh is another important silk-based scaffold for its excellent mechanical properties and good nutrients transport. To prevent cells from leaking out of scaffold after seeding, freeze-dried silk microsponges were incorporated into the macro pores of knitted scaffold. In vitro culture demonstrated that MSCs on scaffolds proliferated vigorously and produced abundant collagen. The transcription levels of ligament-specific genes (collagen I, collagen III, and tenascin-C) also increased significantly with time. The comparison of MSCs and fibroblasts as cell sources for ligament tissue engineering demonstrated that MSC was the most suitable candidate for its vigorous proliferation and ECM production. The MSCs/knitted scaffolds were implanted into rabbits to regenerate ACL in vivo. After 24 weeks, histology observation showed that MSCs were distributed throughout the regenerated ligament and exhibited fibroblast morphology. The key ligament ECM components including collagen I, collagen III, and tenascin-C were produced prominently. Furthermore, direct ligament-bone insertion with typical four zones (bone, mineralized fibrocartilage, fibrocartilage, ligament) was reconstructed, which resembled the native structures of ACL-bone insertion. The tensile strength of regenerated ligament also met the mechanical requirements of daily activities. In conclusion, the results imply that silk scaffold has great potentials in future clinical applications. © 2008 Springer-Verlag.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-3-540-69367-3-10
dc.sourceScopus
dc.subjectligament
dc.subjectMesenchymal stem cells
dc.subjectsilk scaffold
dc.subjecttissue engineering
dc.typeConference Paper
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1007/978-3-540-69367-3-10
dc.description.sourcetitleIFMBE Proceedings
dc.description.volume20 IFMBE
dc.description.page34-37
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

119
checked on May 4, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.