Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/73507
Title: High-throughput circulating tumor cells (CTCs) isolation using inertial forces
Authors: Bhagat, A.A.S.
Hou, H.W.
Huang, S.
Lim, C.T. 
Han, J.
Keywords: Circulating tumor cells
Inertial forces
Spiral channels
Issue Date: 2010
Citation: Bhagat, A.A.S.,Hou, H.W.,Huang, S.,Lim, C.T.,Han, J. (2010). High-throughput circulating tumor cells (CTCs) isolation using inertial forces. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 2 : 1391-1393. ScholarBank@NUS Repository.
Abstract: Continuous flow-based cell separations are indispensable for many biomedical applications and diagnostics including early detection of cancer. Here, we describe a high-throughput cell separation technique for sorting circulating tumor cells (CTCs) from blood using microfluidics. The separation consists of a 2-step process to efficiently isolate CTCs from blood. In the first step, 99% of hematologic cells (erythrocytes (RBCs) and leukocytes) are removed using shear-modulated inertial focusing with >90% CTC recovery. This RBCs depleted sample is then run through a low aspect ratio spiral microchannel to completely isolate the CTCs. The operating principle of this separation method exploits the difference in cell size between CTCs (∼16-20 μm diameter) and other blood cells (RBCs ∼8 μm; leukocytes ∼8-14 μm).
Source Title: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
URI: http://scholarbank.nus.edu.sg/handle/10635/73507
ISBN: 9781618390622
Appears in Collections:Staff Publications

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