Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.638509
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dc.titleCell separation technique in dilectrophoretic chip with bulk electrode
dc.contributor.authorIliescu, C.
dc.contributor.authorTay, F.E.H.
dc.contributor.authorXu, G.
dc.contributor.authorYu, L.
dc.date.accessioned2014-06-19T05:32:48Z
dc.date.available2014-06-19T05:32:48Z
dc.date.issued2006
dc.identifier.citationIliescu, C., Tay, F.E.H., Xu, G., Yu, L. (2006). Cell separation technique in dilectrophoretic chip with bulk electrode. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 6036 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.638509
dc.identifier.issn16057422
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/73241
dc.description.abstractThis paper presents a new technique for separation of two cell populations in a dielectrophoretic chip with bulk silicon electrode. A characteristic of the dielectrophoretic chip is its "sandwich" structure: glass/silicon/glass that generates a unique definition of the microfluidic channel with conductive walls (silicon) and isolating floor and ceiling (glass). The structure confers the opportunity to use the electrodes not only to generate a gradient of the electric field but also to generate a gradient of velocity of the fluid inside the channel. This interesting combination gives rise to a new solution for dielectrophoretic separation of two cell populations. The separation method consists of four steps. First, the microchannel is field with the cells mixture. Second, the cells are trapped in different locations of the microfluidic channel, the cell population which exhibits positive dielectrophoresis is trapped in the area where the distance between the electrodes is the minimum whilst, the other population that exhibit negative dielectrophoresis is trapped where the distance between electrodes is the maximum. In the next step, increasing the flow in the microchannel will result in an increased hydrodynamic force that sweeps the cells trapped by positive dielectrophoresis out of the chip. In the last step, the electric field is removed and the second population is sweep out and collected at the outlet. The device was tested for separation of dead yeast cells from live yeast cells. The paper presents analytical aspects of the separation method a comparative study between different electrode profiles and experimental results.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1117/12.638509
dc.sourceScopus
dc.subjectCells separation
dc.subjectDielectrophoretic chip
dc.subjectDielectrophoretic force
dc.subjectHydrodynamic force
dc.typeConference Paper
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1117/12.638509
dc.description.sourcetitleProgress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.description.volume6036
dc.description.page-
dc.identifier.isiut000236540300011
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