Please use this identifier to cite or link to this item:
Title: Silicon nanopillar substrates for enhancing signal intensity in DNA microarrays
Authors: Murthy, B.R.
Ng, J.K.K.
Selamat, E.S.
Balasubramanian, N.
Liu, W.T. 
Keywords: Dual-substrate pillars
Fluorescence minima
High throughput detection
Nanopillar arrays
Silica chips
SNR enhancement
Issue Date: 1-Dec-2008
Citation: Murthy, B.R., Ng, J.K.K., Selamat, E.S., Balasubramanian, N., Liu, W.T. (2008-12-01). Silicon nanopillar substrates for enhancing signal intensity in DNA microarrays. Biosensors and Bioelectronics 24 (4) : 723-728. ScholarBank@NUS Repository.
Abstract: The use of ordered, high-aspect ratio nanopillar arrays on the surface of silicon-based chips to enhance signal intensity in DNA microarrays is reported. These nanopillars consisting either of a single silicon dioxide substrate or a dual silicon/silicon dioxide substrate are fabricated using deep-UV lithography followed by reactive ion etching. These pillar type arrays provide a three-dimensional high surface-density platform that increases the immobilization capacity of captured probes, enhances target accessibility and reduces background noise interference in DNA microarrays, leading to improved signal-to-noise ratios, sensitivity and specificity. Consequently, it was found that the use of such nanopillars enhanced the hybridization signals by up to seven times as compared to silicon dioxide thin film substrates. In addition, hybridization of synthetic targets to capture probes that contained a single-base variation showed that the perfect matched duplex signals on dual-substrate nanopillars can be up to 23 times higher than the mismatched duplex signals, allowing the targets to be unambiguously identified. These results suggest that the nanopillars, particularly the dual-substrate pillars, are able to enhance the hybridization signals and discrimination power in nucleic acids-based detection, providing an alternative platform for improving the performance of DNA microarrays. © 2008 Elsevier B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/j.bios.2008.06.044
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 10, 2020


checked on Jul 10, 2020

Page view(s)

checked on Jun 28, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.