Please use this identifier to cite or link to this item: https://doi.org/10.1038/ismej.2009.4
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dc.titleQuantitative detection of culturable methanogenic archaea abundance in anaerobic treatment systems using the sequence-specific rRNA cleavage method
dc.contributor.authorNarihiro, T.
dc.contributor.authorTerada, T.
dc.contributor.authorOhashi, A.
dc.contributor.authorWu, J.-H.
dc.contributor.authorLiu, W.-T.
dc.contributor.authorAraki, N.
dc.contributor.authorKamagata, Y.
dc.contributor.authorNakamura, K.
dc.contributor.authorSekiguchi, Y.
dc.date.accessioned2014-06-17T10:15:16Z
dc.date.available2014-06-17T10:15:16Z
dc.date.issued2009-05
dc.identifier.citationNarihiro, T., Terada, T., Ohashi, A., Wu, J.-H., Liu, W.-T., Araki, N., Kamagata, Y., Nakamura, K., Sekiguchi, Y. (2009-05). Quantitative detection of culturable methanogenic archaea abundance in anaerobic treatment systems using the sequence-specific rRNA cleavage method. ISME Journal 3 (5) : 522-535. ScholarBank@NUS Repository. https://doi.org/10.1038/ismej.2009.4
dc.identifier.issn17517362
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67686
dc.description.abstractA method based on sequence-specific cleavage of rRNA with ribonuclease H was used to detect almost all known cultivable methanogens in anaerobic biological treatment systems. To do so, a total of 40 scissor probes in different phylogeny specificities were designed or modified from previous studies, optimized for their specificities under digestion conditions with 32 methanogenic reference strains, and then applied to detect methanogens in sludge samples taken from 6 different anaerobic treatment processes. Among these processes, known aceticlastic and hydrogenotrophic groups of methanogens from the families Methanosarcinaceae, Methanosaetaceae, Methanobacteriaceae, Methanothermaceae and Methanocaldococcaceae could be successfully detected and identified down to the genus level. Within the aceticlastic methanogens, the abundances of mesophilic Methanosaeta accounted for 5.7-48.5 of the total archaeal populations in mesophilic anaerobic processes, and those of Methanosarcina represented 41.7 of the total archaeal populations in thermophilic processes. For hydrogenotrophic methanogens, members of the Methanomicrobiales, Methanobrevibacter and Methanobacterium were detected in mesophilic processes (1.2-17.2), whereas those of Methanothermobacter, Methanothermaceae and Methanocaldococcaceae were detected in thermophilic process (2.0-4.8). Overall results suggested that those hierarchical scissor probes developed could be effective for rapid and possibly on-site monitoring of targeted methanogens in different microbial environments. © 2009 International Society for Microbial Ecology. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/ismej.2009.4
dc.sourceScopus
dc.subjectAnaerobic wastewater treatment process
dc.subjectMethanogen
dc.subjectRNase H method
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.description.doi10.1038/ismej.2009.4
dc.description.sourcetitleISME Journal
dc.description.volume3
dc.description.issue5
dc.description.page522-535
dc.identifier.isiut000265960400003
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