Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.watres.2007.11.037
DC FieldValue
dc.titleIdentification of important microbial populations in the mesophilic and thermophilic phenol-degrading methanogenic consortia
dc.contributor.authorChen, C.-L.
dc.contributor.authorWu, J.-H.
dc.contributor.authorLiu, W.-T.
dc.date.accessioned2014-06-17T10:14:54Z
dc.date.available2014-06-17T10:14:54Z
dc.date.issued2008-04
dc.identifier.citationChen, C.-L., Wu, J.-H., Liu, W.-T. (2008-04). Identification of important microbial populations in the mesophilic and thermophilic phenol-degrading methanogenic consortia. Water Research 42 (8-9) : 1963-1976. ScholarBank@NUS Repository. https://doi.org/10.1016/j.watres.2007.11.037
dc.identifier.issn00431354
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67655
dc.description.abstractActive mesophilic and thermophilic phenol-degrading methanogenic consortia were obtained after an 18-month acclimation and enriching process in the serum bottles, and characterized using the rRNA-based molecular approach. As revealed by cloning, fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP), these two enrichments differed greatly in the community structures. The results for the first time suggest that group TA in the Deltaproteobacteria (88.0% of EUBmix FISH-detectable bacterial cell area) and Pelotomaculum spp. in the Desulfotomaculum family (81.2%) were the predominant fermentative bacteria under mesophilic (37 °C) and thermophilic (55 °C) conditions, respectively. These populations closely associated with mesophilic and thermophilic members of Methanosaetaceae, Methanobacteriaceae and Methanomicrobiales to mineralize phenol as the sole carbon substrate to carbon dioxide and methane. Moreover, these two enrichments could mineralize terephthalate and benzoate. During benzoate degradation in the mesophilic enrichment, a shift in the predominant bacterial population from Deltaproteobacteria group TA to Syntrophus spp. was observed, suggesting Syntrophus-related spp. could have a higher substrate affinity for benzoate. FISH further revealed that member of the Deltaproteobacteria group TA represented more than 68.3% of EUBmix FISH-detectable bacterial cell area in a full-scale mesophilic bioreactor treating phenol-containing wastewaters. © 2007 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.watres.2007.11.037
dc.sourceScopus
dc.subjectAnaerobic degradation
dc.subjectCloning
dc.subjectFISH
dc.subjectMicrobial consortia
dc.subjectPhenol
dc.subjectPhylogeny
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.contributor.departmentTROPICAL MARINE SCIENCE INSTITUTE
dc.description.doi10.1016/j.watres.2007.11.037
dc.description.sourcetitleWater Research
dc.description.volume42
dc.description.issue8-9
dc.description.page1963-1976
dc.description.codenWATRA
dc.identifier.isiut000255825500013
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