Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10544-007-9085-z
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dc.titleCell loss in integrated microfluidic device
dc.contributor.authorZhu, L.
dc.contributor.authorPeh, X.L.
dc.contributor.authorJi, H.M.
dc.contributor.authorTeo, C.Y.
dc.contributor.authorFeng, H.H.
dc.contributor.authorLiu, W.-T.
dc.date.accessioned2014-06-17T10:14:30Z
dc.date.available2014-06-17T10:14:30Z
dc.date.issued2007-10
dc.identifier.citationZhu, L., Peh, X.L., Ji, H.M., Teo, C.Y., Feng, H.H., Liu, W.-T. (2007-10). Cell loss in integrated microfluidic device. Biomedical Microdevices 9 (5) : 745-750. ScholarBank@NUS Repository. https://doi.org/10.1007/s10544-007-9085-z
dc.identifier.issn13872176
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67619
dc.description.abstractCell loss during sample transporting from macro-components to micro-components in integrated microfluidic devices can considerably deteriorate cell detection sensitivity. This intrinsic cell loss was studied and effectively minimized through (a) increasing the tubing diameter connecting the sample storage and the micro-device, (b) applying a hydrodynamic focusing approach for sample delivering to reduce cells contacting and adhesion on the walls of micro-channel and chip inlet; (c) optimizing the filter design with a zigzag arrangement of pillars (13 μm in chamber depth and 0.8 μm in gap) to prolong the effective filter length, and iv) the use of diamond shaped pillar instead of normally used rectangular shape to reduce the gap length between any two given pillar (i.e. pressure drop) at the filter region. Cell trapping and immunofluorescent detection of 12 Giardia lamblia and 12 Cryptosporidium parvumcells in 150 μl solution and 50 MCF-7 breast cancer cells in 150 μl solution was completed within 15 min with trapping efficiencies improved from 79±11%, 50.8±5.5% and 41.3±3.6% without hydrodynamic focusing, respectively, to 90.8±5.8%, 89.8±16.6% and 77.0±9.2% with hydrodynamic focusing. © Springer Science+Business Media, LLC 2007.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s10544-007-9085-z
dc.sourceScopus
dc.subjectCell loss
dc.subjectHydrodynamic focusing
dc.subjectImmnunofluorescent assay
dc.subjectLab-on-a-chip
dc.subjectMicrofluidic device
dc.subjectTrapping efficiency
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.description.doi10.1007/s10544-007-9085-z
dc.description.sourcetitleBiomedical Microdevices
dc.description.volume9
dc.description.issue5
dc.description.page745-750
dc.description.codenBMICF
dc.identifier.isiut000248975100014
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