Please use this identifier to cite or link to this item:
DC FieldValue
dc.titleThe physiological relevance of wet versus dry differential scanning calorimetry for biomaterial evaluation: A technical note
dc.contributor.authorZeugolis, D.I.
dc.contributor.authorRaghunath, M.
dc.identifier.citationZeugolis, D.I., Raghunath, M. (2010-10). The physiological relevance of wet versus dry differential scanning calorimetry for biomaterial evaluation: A technical note. Polymer International 59 (10) : 1403-1407. ScholarBank@NUS Repository.
dc.description.abstractCollagen and its denatured form, gelatin, have been extensively used as scaffolds for tissue engineering and tissue repair applications. Denaturation temperature, commonly measured using differential scanning calorimetry (DSC), for biomaterial applications is a significant physical property that will determine the stability of a potential implant at body temperature. In order to imitate a clinical setting, DSC should be run under fully hydrated conditions. We show here that for hydrophobic polymers such as poly(ε-caprolactone) and chitosan there is no significant difference between dry and wet DSC operation (p > 0.05). In contrast, for hydrophilic polymers such as collagen, gelatin, poly(ethylene glycol) (40 kDa) and poly(ethylene oxide) (900 kDa) significant differences occur between measurements in the dry and the wet state (p < 0.0011). Moreover, we demonstrate that only when wet DSC is carried out are we able to separate the unique crystalline structure of collagen from its randomly coiled heat-denatured by-product gelatin (p < 0.0005). We therefore recommend running DSC under fully hydrated conditions when the function and properties of a biomaterial are under investigation. © 2010 Society of Chemical Industry.
dc.subjectDifferential scanning calorimetry
dc.subjectHydrophilic and hydrophobic polymers
dc.description.sourcetitlePolymer International
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.