Please use this identifier to cite or link to this item:
https://doi.org/10.1002/mabi.200900081
Title: | Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells | Authors: | Loh, X.J. Gong, J. Sakuragi, M. Kitajima, T. Liu, M. Li, J. Ito, Y. |
Keywords: | Embryonic stem cell Functional coating Polyhydroxybutyric acid Surface modification Thermally responsive material |
Issue Date: | 10-Nov-2009 | Citation: | Loh, X.J., Gong, J., Sakuragi, M., Kitajima, T., Liu, M., Li, J., Ito, Y. (2009-11-10). Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. Macromolecular Bioscience 9 (11) : 1069-1079. ScholarBank@NUS Repository. https://doi.org/10.1002/mabi.200900081 | Abstract: | A thermoresponsive substrate based on a triblock copolymer, poly(N-isopropylacrylamide)-block-poly[(R)-3-hydroxybutyrate] -block-poly(N-isopropylacrylamide) (PNIPAAm-PHB-PNI-PAAm), co-coated with gelatin, was developed for the culture and non-enzymatic recovery of mouse embryonic stem cells. After culture, the cells could be detached by cooling at 4°C for 20 min without trypsin digestion. The embryonic stem cells remained undifferentiated after culture on the gelatin/copolymer-coated surfaces, similar to standard culture techniques. Overall, the triblock copolymer coating was superior to the PNI-PAAm homopolymer coating in terms of supporting better cell growth, being more stable, presenting a more homogeneous surface coating, and maintaining pluripotency of the embryonic stem cells. (Figure Presented) © 2009 Wiley-VCH Verlag GmbH & Co. KGaA. | Source Title: | Macromolecular Bioscience | URI: | http://scholarbank.nus.edu.sg/handle/10635/67294 | ISSN: | 16165187 | DOI: | 10.1002/mabi.200900081 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
SCOPUSTM
Citations
62
checked on Mar 7, 2021
WEB OF SCIENCETM
Citations
57
checked on Feb 26, 2021
Page view(s)
75
checked on Mar 1, 2021
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.