Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.toxicon.2008.06.003
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dc.titlePurification and N-terminal sequence of a serine proteinase-like protein (BMK-CBP) from the venom of the Chinese scorpion (Buthus martensii Karsch)
dc.contributor.authorGao, R.
dc.contributor.authorZhang, Y.
dc.contributor.authorGopalakrishnakone, P.
dc.date.accessioned2014-06-17T09:46:11Z
dc.date.available2014-06-17T09:46:11Z
dc.date.issued2008-08-01
dc.identifier.citationGao, R., Zhang, Y., Gopalakrishnakone, P. (2008-08-01). Purification and N-terminal sequence of a serine proteinase-like protein (BMK-CBP) from the venom of the Chinese scorpion (Buthus martensii Karsch). Toxicon 52 (2) : 348-353. ScholarBank@NUS Repository. https://doi.org/10.1016/j.toxicon.2008.06.003
dc.identifier.issn00410101
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67245
dc.description.abstractA serine proteinase-like protein was isolated from the venom of Chinese red scorpion (Buthus martensii Karsch) by combination of gel filtration, ion-exchange and reveres-phase chromatography and named BMK-CBP. The apparent molecular weight of BMK-CBP was identified as 33 kDa by SDS-PAGE under non-reducing condition. The sequence of N-terminal 40 amino acids was obtained by Edman degradation. The sequence shows highest similarity to proteinase from insect source. When tested with commonly used substrates of proteinase, no significant hydrolytic activity was observed for BMK-CBP. The purified BMK-CBP was found to bind to the cancer cell line MCF-7 and the cell binding ability was dose-dependent. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.toxicon.2008.06.003
dc.sourceScopus
dc.subjectCell binding
dc.subjectPurification
dc.subjectScorpion venom
dc.subjectSerine proteinase
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.toxicon.2008.06.003
dc.description.sourcetitleToxicon
dc.description.volume52
dc.description.issue2
dc.description.page348-353
dc.description.codenTOXIA
dc.identifier.isiut000259461400018
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