Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12195-011-0186-7
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dc.titleHuman corneal keratocyte response to micro-and nano-gratings on chitosan and PDMS
dc.contributor.authorKoo, S.
dc.contributor.authorAhn, S.J.
dc.contributor.authorZhang, H.
dc.contributor.authorWang, J.C.
dc.contributor.authorYim, E.K.F.
dc.date.accessioned2014-06-17T09:44:15Z
dc.date.available2014-06-17T09:44:15Z
dc.date.issued2011-09
dc.identifier.citationKoo, S., Ahn, S.J., Zhang, H., Wang, J.C., Yim, E.K.F. (2011-09). Human corneal keratocyte response to micro-and nano-gratings on chitosan and PDMS. Cellular and Molecular Bioengineering 4 (3) : 399-410. ScholarBank@NUS Repository. https://doi.org/10.1007/s12195-011-0186-7
dc.identifier.issn18655025
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67086
dc.description.abstractCorneal stroma accounts for major refractive power and the transparency of cornea. This transparency is contributed mainly by the highly ordered arrangement of the extracellular matrix (ECM), particularly the collagen fibrils. Shortage and complications of cornea transplantation have remained an issue for decades. Attempts to produce tissueengineered cornea are met with challenges, one of which is to induce alignment of stromal ECM produced by keratocytes. In this study, human corneal keratocyte response toward substrate topography on two different materials was examined. Primary human keratocytes were cultured on chitosan and polydimethylsiloxane (PDMS) surface patterned with anisotopic topography of gratings in various widths. Cell response in the form of alignment, elongation and proliferation were analyzed. Collagen I deposition was imaged and the gene expression analysis of keratocytes cultured on PDMS were studied. On both chitosan and PDMS, keratocytes were found to be aligned and elongated in grating direction. Proliferation rate was reduced as grating width decreased. Aldehyde-3-dehydrogenase (ALDH3) expression was increased on nanogratings. Deposited collagen I followed the keratocyte orientation, which was aligned along the direction of gratings. In conclusion, keratocytes responded similarly to topographical cues when presented on materials with different surface chemistry and stiffness and nanogratings were observed to be more efficient in inducing these responses than microgratings. © 2011 Biomedical Engineering Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s12195-011-0186-7
dc.sourceScopus
dc.subjectChitosan
dc.subjectCorneal stroma
dc.subjectExtracellular matrix alignment
dc.subjectKeratocyte
dc.subjectNanotopography
dc.subjectResponse to topography
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1007/s12195-011-0186-7
dc.description.sourcetitleCellular and Molecular Bioengineering
dc.description.volume4
dc.description.issue3
dc.description.page399-410
dc.identifier.isiut000297866300009
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