Please use this identifier to cite or link to this item: https://doi.org/10.1089/scd.2008.0157
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dc.titleHeparan sulfate mediates the proliferation and differentiation of rat mesenchymal stem cells
dc.contributor.authorDombrowski, C.
dc.contributor.authorSong, S.J.
dc.contributor.authorChuan, P.
dc.contributor.authorLim, X.
dc.contributor.authorSusanto, E.
dc.contributor.authorSawyer, A.A.
dc.contributor.authorWoodruff, M.A.
dc.contributor.authorHutmacher, D.W.
dc.contributor.authorNurcombe, V.
dc.contributor.authorCool, S.M.
dc.date.accessioned2014-06-17T09:44:10Z
dc.date.available2014-06-17T09:44:10Z
dc.date.issued2009-05-01
dc.identifier.citationDombrowski, C., Song, S.J., Chuan, P., Lim, X., Susanto, E., Sawyer, A.A., Woodruff, M.A., Hutmacher, D.W., Nurcombe, V., Cool, S.M. (2009-05-01). Heparan sulfate mediates the proliferation and differentiation of rat mesenchymal stem cells. Stem Cells and Development 18 (4) : 661-670. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2008.0157
dc.identifier.issn15473287
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67079
dc.description.abstractThe growth and differentiation of mesenchymal stem cells (MSCs) is controlled by various growth factors, the activities of which can be modulated by heparan sulfates (HSs). We have previously noted the necessity of sulfated glycosaminoglycans for the fibroblast growth factor type 2 (FGF-2)-stimulated differentiation of osteoprogenitor cells. Here we show that exogenous application of HS to cultures of primary rat MSCs stimulates their proliferation, leading to increased expression of osteogenic markers and enhanced bone nodule formation. FGF-2 can also increase the proliferation, and osteogenic differentiation of rat bone marrow stem cells (rMSCs) when applied exogenously during their linear growth. However, as opposed to exogenous HS, the continuous use of FGF-2 during in vitro differentiation completely blocked rMSC mineralization. We show that the effects of both FGF-2 and HS are mediated through FGF receptor 1 (FGFR1) and that inhibition of signaling through this receptor arrests cell growth, resulting in the cells being unable to reach the critical density necessary to induce differentiation. Blocking FGFR1 signaling in postconfluent osteogenic cultures significantly increased calcium deposition. Taken together our data suggest that FGFR1 signaling plays an important role during osteogenic differentiation, first by stimulating cell growth that is closely followed by an inhibitory effect once the cells have reached confluence. It also confirms the importance of HS as a coreceptor for the signaling of endogenous FGF-2 and suggests that purified glycosaminoglycans may be attractive alternatives to growth factors for improved ex vivo growth and differentiation of MSCs. © 2009 Mary Ann Liebert, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/scd.2008.0157
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1089/scd.2008.0157
dc.description.sourcetitleStem Cells and Development
dc.description.volume18
dc.description.issue4
dc.description.page661-670
dc.description.codenSCDTA
dc.identifier.isiut000265996400013
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