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Title: Functionalization of chitosan via atom transfer radical polymerization for gene delivery
Authors: Ping, Y.
Liu, C.-D.
Tang, G.-P.
Li, J.-S.
Li, J. 
Yang, W.-T.
Xu, F.-J.
Issue Date: 23-Sep-2010
Citation: Ping, Y., Liu, C.-D., Tang, G.-P., Li, J.-S., Li, J., Yang, W.-T., Xu, F.-J. (2010-09-23). Functionalization of chitosan via atom transfer radical polymerization for gene delivery. Advanced Functional Materials 20 (18) : 3106-3116. ScholarBank@NUS Repository.
Abstract: It is of crucial importance to modify chitosan-based polysaccharides in the designing of biomedical materials. In this work, atom transfer radical polymerization (ATRP) was employed to functionalize chitosan in a well-controlled manner. A series of new degradable cationic polymers (termed as PDCS) composed of biocompatible chitosan backbones and poly((2-dimethyl amino)ethyl methacrylate) (P(DMAEMA)) side chains of different length were designed as highly efficient gene vectors via ATRP. These vectors, termed as PDCS, exhibited good ability to condense plasmid DNA (pDNA) into nanoparticles with positive charge at nitrogen/phosphorus (N/P) ratios of 4 or higher. All PDCS vectors could well protect the condensed DNA from enzymatic degradation by DNase I and they displayed high level oftransfectivity in both COS7, HEK293 and HepG2 cell lines. Most importantly, in comparison with high-molecular-weight P(DMAEMA) and 'gold-standard' PEI (25 kDa), the PDCS vectors showed considerable buffering capacity in the pH range of 7.4 to 5, and were capable of mediating much more efficient gene transfection at low N/P ratios. At their own optimal N/P ratios for trasnsfection, the PDCS/pDNA complexes showed much lower cytotoxicity. All the PDCS vectors were readily to be degradable in the presence of lysozyme at physiological conditions in vitro. These well-defined PDCS polymers have great potentials as efficient gene vectors in future gene therapy. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA.
Source Title: Advanced Functional Materials
ISSN: 1616301X
DOI: 10.1002/adfm.201000177
Appears in Collections:Staff Publications

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