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|Title:||Elliptically polarized coherent anti-Stokes Raman scattering microscopy||Authors:||Lu, F.
|Issue Date:||1-Dec-2008||Citation:||Lu, F.,Zheng, W.,Huang, Z. (2008-12-01). Elliptically polarized coherent anti-Stokes Raman scattering microscopy. Optics Letters 33 (23) : 2842-2844. ScholarBank@NUS Repository.||Abstract:||We report an approach to coherent anti-Stokes Raman scattering (CARS) microscopy that significantly suppresses the nonresonant background for high-contrast vibrational imaging. We employ an elliptically polarized pump field with an aspect ratio of 1 : √3 of the ellipse combined with a linearly polarized Stokes field for elliptically polarized CARS (EP-CARS) microscopy. The EP-CARS technique yields a 6-7-fold improvement in signal-to-background ratios compared with normal CARS, while providing an approximately 1.25-fold improvement in resonant CARS signal compared with normal polarization-sensitive CARS microscopy. We demonstrate this technique by imaging 1.5 μm polystyrene beads in water and lipid droplets in unstained fibroblast cells. © 2008 Optical Society of America.||Source Title:||Optics Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/67026||ISSN:||01469592|
|Appears in Collections:||Staff Publications|
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