Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ab.2010.11.031
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dc.titleDetection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis
dc.contributor.authorCheng, J.
dc.contributor.authorYim, O.-S.
dc.contributor.authorLow, P.-S.
dc.contributor.authorTay, S.K.H.
dc.contributor.authorYap, E.P.H.
dc.contributor.authorLai, P.-S.
dc.date.accessioned2014-06-17T09:43:12Z
dc.date.available2014-06-17T09:43:12Z
dc.date.issued2011-03-01
dc.identifier.citationCheng, J., Yim, O.-S., Low, P.-S., Tay, S.K.H., Yap, E.P.H., Lai, P.-S. (2011-03-01). Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis. Analytical Biochemistry 410 (1) : 158-160. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2010.11.031
dc.identifier.issn00032697
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/66998
dc.description.abstractHeteroduplex formation, required for the complete detection of hemi/homozygotes using high-resolution melting analysis, can be induced either by pre-PCR mixing of genomic DNAs or by post-PCR mixing of PCR products from unknown and reference samples. This study investigates the effects of both methods using two single nucleotide polymorphisms in X-linked DMD gene. The results show that both methods resulted in the same effect when mixing samples with the same gene copy number. Mixing samples with different gene copy numbers has not been previously explored and we show that post-PCR mixing is insensitive to gene copy number differences as compared to pre-PCR mixing. © 2010 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ab.2010.11.031
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.ab.2010.11.031
dc.description.sourcetitleAnalytical Biochemistry
dc.description.volume410
dc.description.issue1
dc.description.page158-160
dc.description.codenANBCA
dc.identifier.isiut000286711300024
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