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Title: Colonization and osteogenic differentiation of different stem cell sources on electrospun nanofiber meshes
Authors: Kolambkar, Y.M.
Peister, A.
Ekaputra, A.K. 
Hutmacher, D.W.
Guldberg, R.E.
Issue Date: 1-Oct-2010
Citation: Kolambkar, Y.M., Peister, A., Ekaputra, A.K., Hutmacher, D.W., Guldberg, R.E. (2010-10-01). Colonization and osteogenic differentiation of different stem cell sources on electrospun nanofiber meshes. Tissue Engineering - Part A 16 (10) : 3219-3230. ScholarBank@NUS Repository.
Abstract: Numerous challenges remain in the successful clinical translation of cell-based therapies for musculoskeletal tissue repair, including the identification of an appropriate cell source and a viable cell delivery system. The aim of this study was to investigate the attachment, colonization, and osteogenic differentiation of two stem cell types, human mesenchymal stem cells (hMSCs) and human amniotic fluid stem (hAFS) cells, on electrospun nanofiber meshes. We demonstrate that nanofiber meshes are able to support these cell functions robustly, with both cell types demonstrating strong osteogenic potential. Differences in the kinetics of osteogenic differentiation were observed between hMSCs and hAFS cells, with the hAFS cells displaying a delayed alkaline phosphatase peak, but elevated mineral deposition, compared to hMSCs. We also compared the cell behavior on nanofiber meshes to that on tissue culture plastic, and observed that there is delayed initial attachment and proliferation on meshes, but enhanced mineralization at a later time point. Finally, cell-seeded nanofiber meshes were found to be effective in colonizing three-dimensional scaffolds in an in vitro system. This study provides support for the use of the nanofiber mesh as a model surface for cell culture in vitro, and a cell delivery vehicle for the repair of bone defects in vivo. © 2010 Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part A
ISSN: 19373341
DOI: 10.1089/ten.tea.2010.0004
Appears in Collections:Staff Publications

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