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Title: Collective migration behaviors of human breast cancer cells in 2D
Authors: Mendoz, E.
Lim, C.T. 
Keywords: 2D collective cell migration
Cancer metastasis
Cell mechanics
Cell-cell adhesion
Lamellipod formation
Issue Date: Sep-2011
Citation: Mendoz, E., Lim, C.T. (2011-09). Collective migration behaviors of human breast cancer cells in 2D. Cellular and Molecular Bioengineering 4 (3) : 411-426. ScholarBank@NUS Repository.
Abstract: Cancer related deaths are mainly attributed to the spread of cancer cells to distant organs to form secondary tumors by a process called metastasis. Migration is one of the factors among many others that are strongly implicated in this phenomenon. Here we studied the migratory behavior of benign (MCF-10A), non-invasive malignant (MCF-7) and highly-invasive malignant (MDA-MB-231) tumor cell lines which are derivatives of ductal epithelium of the human breast, using a modified ring cell migration assay technique. Time lapse phase contrast video microscopy was used to monitor migration on both culture coated and Collagen-IV coated surfaces. Individual cells were tracked for 24 h and observations were made on the morphological development of the lamellipods. Analyses show that an intact intercellular adhesion coupled with unidirectional lamellipod formation in benign cells help to coordinate migratory behavior when compared against the malignant variants. Among the cancer cell lines, the non-invasive malignant cells had shorter migratory distances and exhibited pseudo-coordinated behavior due to altered or defective lamellipod morphology and intercellular adhesion, while the highly invasive malignant cells displayed individualistic and chaotic movements with little or no intercellular adhesion. Collagen-IV coating increased the migration rates of the highly invasive cell lines, while the effect was less pronounced on the other two cell lines. © 2011 Biomedical Engineering Society.
Source Title: Cellular and Molecular Bioengineering
ISSN: 18655025
DOI: 10.1007/s12195-011-0193-8
Appears in Collections:Staff Publications

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