Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(01)00725-7
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dc.titleContribution of axial dispersion to band spreading in perfusion chromatography
dc.contributor.authorGeng, A.
dc.contributor.authorLoh, K.-C.
dc.date.accessioned2014-06-17T08:31:08Z
dc.date.available2014-06-17T08:31:08Z
dc.date.issued2001-05-18
dc.identifier.citationGeng, A., Loh, K.-C. (2001-05-18). Contribution of axial dispersion to band spreading in perfusion chromatography. Journal of Chromatography A 918 (1) : 37-46. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(01)00725-7
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/66505
dc.description.abstractA new interpretation of the band spreading data in perfusion chromatography is proposed by investigating the relative importance of axial dispersion in perfusive beds. Elution chromatography of proteins (bovine serum albumin and lysozyme) under non-retained conditions on two kinds of reversed-phase perfusive supports (POROS R1/H and POROS R2/H), which have different pore structures, were carried out to obtain the axial dispersion data. The Knox equation and some empirical correlations for dispersion coefficients in porous media were applied to correlate the experimental data. The influences of particle properties, solute molecular sizes and flow velocity on the dispersion coefficient were elucidated. Axial dispersion was recognised to be the main contributor to peak broadening in perfusion chromatography. The dependence of the height equivalent to a theoretical plate on flow-rate was found to be the result of the velocity dependence of the axial dispersion. The dispersion coefficient in a perfusive column can be well represented both by a power-law relationship and a correlation derived based on stochastic theory. Pursuant to these, it was found that pore size distribution of the perfusive particles and solute molecular size are important parameters, which influenced the dispersion results significantly. © 2001 Elsevier Science B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0021-9673(01)00725-7
dc.sourceScopus
dc.subjectAxial dispersion
dc.subjectBand spreading
dc.subjectPerfusion chromatography
dc.subjectProteins
dc.typeArticle
dc.contributor.departmentCHEMICAL & ENVIRONMENTAL ENGINEERING
dc.description.doi10.1016/S0021-9673(01)00725-7
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume918
dc.description.issue1
dc.description.page37-46
dc.description.codenJCRAE
dc.identifier.isiut000168678800004
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