Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.71.10.6453-6457.2005
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dc.titleEmission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
dc.contributor.authorLiu, W.-T.
dc.contributor.authorWu, J.-H.
dc.contributor.authorLi, E.S.-Y.
dc.contributor.authorSelamat, E.S.
dc.date.accessioned2014-06-17T08:17:45Z
dc.date.available2014-06-17T08:17:45Z
dc.date.issued2005-10
dc.identifier.citationLiu, W.-T., Wu, J.-H., Li, E.S.-Y., Selamat, E.S. (2005-10). Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies. Applied and Environmental Microbiology 71 (10) : 6453-6457. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.71.10.6453-6457.2005
dc.identifier.issn00992240
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/65526
dc.description.abstractThe effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/AEM.71.10.6453-6457.2005
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCIVIL ENGINEERING
dc.description.doi10.1128/AEM.71.10.6453-6457.2005
dc.description.sourcetitleApplied and Environmental Microbiology
dc.description.volume71
dc.description.issue10
dc.description.page6453-6457
dc.description.codenAEMID
dc.identifier.isiut000232504000106
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