Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.03.034
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dc.titleWhole-cell based solvent-free system for one-pot production of biodiesel from waste grease
dc.contributor.authorLi, A.
dc.contributor.authorNgo, T.P.N.
dc.contributor.authorYan, J.
dc.contributor.authorTian, K.
dc.contributor.authorLi, Z.
dc.date.accessioned2014-06-17T07:51:25Z
dc.date.available2014-06-17T07:51:25Z
dc.date.issued2012-06
dc.identifier.citationLi, A., Ngo, T.P.N., Yan, J., Tian, K., Li, Z. (2012-06). Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresource Technology 114 : 725-729. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.03.034
dc.identifier.issn09608524
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64796
dc.description.abstractA whole-cell based solvent-free system was developed for efficient conversion of waste grease to biodiesel via one-pot esterification and transesterification. By isolation and screening of lipase-producing strains from soil, Serratia marcescens YXJ-1002 was discovered for the biotransformation of grease to biodiesel. The lipase (SML) from this strain was cloned and expressed in Escherichia coli as an intracellular enzyme, showing 6 times higher whole-cell based hydrolysis activity than that of wild type strain. The recombinant cells were used for biodiesel production from waste grease in one-pot reactions containing no solvent with the addition of methanol in several small portions, and 97% yield of biodiesel (FAME) was achieved under optimized conditions. In addition, the whole-cell biocatalysts showed excellent reusability, retaining 74% productivity after 4 cycles. The developed system, biocatalyst, and process enable the efficient, low-cost, and green production of biodiesel from waste grease, providing with a potential industrial application. © 2012 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biortech.2012.03.034
dc.sourceScopus
dc.subjectBiodiesel
dc.subjectBiotransformation
dc.subjectFree fatty acid
dc.subjectGrease
dc.subjectWhole-cell
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biortech.2012.03.034
dc.description.sourcetitleBioresource Technology
dc.description.volume114
dc.description.page725-729
dc.description.codenBIRTE
dc.identifier.isiut000305035900105
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