Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/64706
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dc.titleThe facile synthesis of an aldehyde-containing graft copolymer membrane for covalent protein capture with retention of protein functionality
dc.contributor.authorYong, J.K.J.
dc.contributor.authorXiao, Y.
dc.contributor.authorChung, T.-S.
dc.date.accessioned2014-06-17T07:50:20Z
dc.date.available2014-06-17T07:50:20Z
dc.date.issued2010-03-19
dc.identifier.citationYong, J.K.J., Xiao, Y., Chung, T.-S. (2010-03-19). The facile synthesis of an aldehyde-containing graft copolymer membrane for covalent protein capture with retention of protein functionality. Journal of Chromatography A 1217 (12) : 1904-1911. ScholarBank@NUS Repository.
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64706
dc.description.abstractThe immobilization of biomolecules onto an insoluble carrier surface has always been a subject of great interest to enhance their resistance to pH and temperature, which aids in an increased activity lifespan as well as easy reuse of the said biomolecules. However, traditional methods are only able to provide single-layer biomolecular binding and require multiple chemical reactions to prepare the final substrate before the immobilization can be carried out properly. Here we report a facile one-step chemical synthesis of a new aldehyde-bearing graft copolymer via atom transfer radical polymerization (ATRP) for covalent protein capture in a multilayered approach to covalently capture bovine serum albumin (BSA) onto a polymeric membrane. The resultant protein-bound membrane illustrated the retention of BSA's stereoselective discrimination ability by binding to an excess of 2 mol of tryptophan/mol of BSA and demonstrated an enantioresolution of a 0.184 mM racemic tryptophan mixture with a time-averaged-separation factor of 2.9. © 2010 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.chroma.2010.01.051
dc.sourceScopus
dc.subjectAtom transfer radical polymerization
dc.subjectMembrane chromatography
dc.subjectProtein adsorption
dc.subjectTryptophan enantioresolution
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume1217
dc.description.issue12
dc.description.page1904-1911
dc.description.codenJCRAE
dc.identifier.isiut000275680600006
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