Please use this identifier to cite or link to this item:
https://doi.org/10.1002/adsc.200900210
DC Field | Value | |
---|---|---|
dc.title | Regio- and stereoselective allylic hydroxylation of D-limonene to (+)-trans-carveol with cellulosimicrobium cellulans EB-8-4 | |
dc.contributor.author | Wang, Z. | |
dc.contributor.author | Lie, F. | |
dc.contributor.author | Lim, E. | |
dc.contributor.author | Li, K. | |
dc.contributor.author | Li, Z. | |
dc.date.accessioned | 2014-06-17T07:47:59Z | |
dc.date.available | 2014-06-17T07:47:59Z | |
dc.date.issued | 2009-08 | |
dc.identifier.citation | Wang, Z., Lie, F., Lim, E., Li, K., Li, Z. (2009-08). Regio- and stereoselective allylic hydroxylation of D-limonene to (+)-trans-carveol with cellulosimicrobium cellulans EB-8-4. Advanced Synthesis and Catalysis 351 (11-12) : 1849-1856. ScholarBank@NUS Repository. https://doi.org/10.1002/adsc.200900210 | |
dc.identifier.issn | 16154150 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/64502 | |
dc.description.abstract | Cellulosimicrobium cellulans EB-8-4 was discovered by screening of microorganisms as a pow-erful catalyst for the regio- and stereoselective allylic hydroxylation of D-limonene to (+)-trans-carveol that is a useful and valuable fragrance and flavour compound. Cells of strain EB-8-4 were easily obtained, demonstrated more than 99% regio- and stereoselectivity, showed a specific hydroxylation activity of 4.0 U/g cdw (cell dry weight), and accepted 62 mM D-limonene without inhibition. The hydroxylation was possibly catalyzed by an nicotinamide adenine dinucleotide (NADH)-dependent oxygenase involved in the degradation of aromatic ring during cell growth. 13.4 mM of (+)-trans-carveol were obtained by biohydroxylation of D-limonene with resting cells of C. cellulans EB-8-4, thus being 11 times higher than that obtained with the best biocatalyst known thus far. High conversion and high yield were obtained in the biohydroxylation of 11.6 mM of D-limonene with the resting cells as catalyst in a closed shaking flask, giving 10 mM of (+)-irans-carveol, and 0.30 mM of carvone as the only by-product. Thus, a unique biocatalyst for the regio- and stereoselective allylic hydroxylation of D-limonene and an efficient synthesis of natural identical (.+)-trans-carveol by biohydroxylation have been developed. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/adsc.200900210 | |
dc.source | Scopus | |
dc.subject | Biotransformations | |
dc.subject | D-limonene | |
dc.subject | Enzyme catalysis | |
dc.subject | Hydroxylation | |
dc.subject | Terpenoids | |
dc.subject | Trans-carveol | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1002/adsc.200900210 | |
dc.description.sourcetitle | Advanced Synthesis and Catalysis | |
dc.description.volume | 351 | |
dc.description.issue | 11-12 | |
dc.description.page | 1849-1856 | |
dc.description.coden | JPCHF | |
dc.identifier.isiut | 000269583900024 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.