Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.a.31257
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dc.titleProteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: A model of liver tissue engineering system
dc.contributor.authorXin, H.Z.
dc.contributor.authorSeng, K.G.
dc.contributor.authorWang, C.-H.
dc.contributor.authorTong, Y.W.
dc.date.accessioned2014-06-17T07:47:41Z
dc.date.available2014-06-17T07:47:41Z
dc.date.issued2007-11-01
dc.identifier.citationXin, H.Z., Seng, K.G., Wang, C.-H., Tong, Y.W. (2007-11-01). Proteins combination on PHBV microsphere scaffold to regulate Hep3B cells activity and functionality: A model of liver tissue engineering system. Journal of Biomedical Materials Research - Part A 83 (3) : 606-616. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.a.31257
dc.identifier.issn15493296
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64476
dc.description.abstractThe synergistic effects of extracellular matrix (ECM) protein combinations on Hep3B cell proliferation and functions are studied herein. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microspheres were covalently conjugated with three types of proteins, collagen (type I), laminin, and fibronectin, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide cross linkers. Successful conjugations of protein molecules were verified by the presence of nitrogen peaks in X-ray photoelectron spectroscopy. The densities of grafted proteins were quantified using Micro-BCA kit. A human hepatoma cell line, Hep3B, was then cultured in vitro on the ECM proteins-modified microspheres for 2 weeks. Cell proliferation was estimated using MTT method, and two hepatic functions, albumin secretion and P-450 activity, were evaluated using ELISA and EROD assays, respectively. The results indicated that combination of the three ECM proteins on microsphere surfaces has a significant effect on the proliferation of Hep3B cells, thus better mimicking the in vivo environment for liver tissue engineering. © 2007 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jbm.a.31257
dc.sourceScopus
dc.subjectLiver tissue engineering
dc.subjectMicrosphere
dc.subjectPHBV
dc.subjectProtein
dc.subjectSurface conjugation
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/jbm.a.31257
dc.description.sourcetitleJournal of Biomedical Materials Research - Part A
dc.description.volume83
dc.description.issue3
dc.description.page606-616
dc.description.codenJBMRC
dc.identifier.isiut000250742700004
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