Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.03.041
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dc.titlePentablock copolymers of poly(ethylene glycol), poly((2-dimethyl amino)ethyl methacrylate) and poly(2-hydroxyethyl methacrylate) from consecutive atom transfer radical polymerizations for non-viral gene delivery
dc.contributor.authorXu, F.-J.
dc.contributor.authorLi, H.
dc.contributor.authorLi, J.
dc.contributor.authorZhang, Z.
dc.contributor.authorKang, E.-T.
dc.contributor.authorNeoh, K.-G.
dc.date.accessioned2014-06-17T07:46:34Z
dc.date.available2014-06-17T07:46:34Z
dc.date.issued2008-07
dc.identifier.citationXu, F.-J., Li, H., Li, J., Zhang, Z., Kang, E.-T., Neoh, K.-G. (2008-07). Pentablock copolymers of poly(ethylene glycol), poly((2-dimethyl amino)ethyl methacrylate) and poly(2-hydroxyethyl methacrylate) from consecutive atom transfer radical polymerizations for non-viral gene delivery. Biomaterials 29 (20) : 3023-3033. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.03.041
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64382
dc.description.abstractWell-defined pentablock copolymers (PBPs) of P(HEMA)-b-P(DMAEMA)-b-PEG-b-P(DMAEMA)-b-P(HEMA) (in which PEG = poly(ethylene glycol), P(DMAEMA) = poly((2-dimethyl amino)ethyl methacrylate), and P(HEMA) = poly(2-hydroxyethyl methacrylate)), with different block lengths of P(DMAEMA), for non-viral gene delivery were prepared via consecutive atom transfer radical polymerizations (ATRPs) from the same di-2-bromoisobutyryl-terminated PEG (Br-PEG-Br) center block. The PBPs demonstrate good ability to condense plasmid DNA (pDNA) into 100-160 nm size nanoparticles with positive zeta potentials of 25-35 mV at PBPs/pDNA weight ratios of 5-25. The PBPs exhibit very low in vitro cytotoxicity and excellent gene transfection efficiency in HEK293 and COS7 cells. In particular, the transfection efficiencies of all the PBPs in HEK293 cells are comparable to, or higher than those of polyethylenimine (PEI, 25 kDa) at most weight ratios. The ability of the copolymers to condense plasmid DNA and the transfection efficiency of the resulting complexes are dependent on the chain length of P(DMAEMA) blocks. In addition to reducing the cytotoxicity and increasing the stability of the plasmid complexes, the PEG center block and the short P(HEMA) end blocks also help to enhance the gene transfection efficiency. Thus, the approach to well-defined block copolymers via ATRP provides a versatile means for tailoring the structure of non-viral gene vectors to meet the requirements of low cytotoxicity, good stability and high transfection capability for gene therapy applications. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.03.041
dc.sourceScopus
dc.subjectATRP
dc.subjectGene delivery
dc.subjectP(DMAEMA)
dc.subjectP(HEMA)
dc.subjectPEG
dc.subjectPentablock copolymers
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.biomaterials.2008.03.041
dc.description.sourcetitleBiomaterials
dc.description.volume29
dc.description.issue20
dc.description.page3023-3033
dc.description.codenBIMAD
dc.identifier.isiut000256655100010
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